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Malignant tumor has become a major public health problem, and it is the leading cause of death in China. Malignant tumor patients are usually accompanied with serious emotional or psychological problems. Epidemiological studies have shown that adverse emotions can affect the occurrence and progression of malignant tumors and the survival time of patients, which leads to the death of cancer patients accounting for about 40% of cancer-related death. As the medical model transforms to bio-psycho-social model, the effects of emotions and psychological factors on the initiation and development of malignant tumors have attracted wide attention in medical field, and cancer has been classified as a kind of psychosomatic disease. This paper reviews the relationship of moods with tumorigenesis, development, prognosis and treatment as well as the underlying mechanisms, in order to remind the cancer patients in tumor therapy should pay attention to the care of emotions, and to prevent disease via psychotherapy.
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Objective:To evaluate the synergistic antibacterial effects of lysozyme with ethylenediami-netetraacetic acid disodium salt (EDTA-2Na) on Enterococcus faecalis (E.faecalis) and Porphyromonas endodontalis ( P.endodontalis) .Methods:E.faecalis and P.endodontalis were cultured and adjusted to 108 CFU/mL.Then 0.3, 0.5, 1, 2, 5, 10, 50, 100, 150 and 300 g/L of lysozyme were prepared with deionized water;and the lysozyme solutions were mixed with 0.5, 1.0, 2.0 g/L of EDTA-2Na, re-spectively.The bacteria and lysosome with/without EDTA-2Na interacted for 15 min, then water-soluble tetrazolium (WST) working solution was added and the activity of the bacteria was calculated by mea-suring optical densities at 450 nm and 630 nm with microplate spectrophotometer .Results:Regarding the pure lysozyme from 0.5 g/L to 150 g/L, more E.faecalis and P.endodontalis were inhibited when the concentration of lysozyme was higher , especially for E.faecalis.There was synergistic effect of lysozyme with EDTA-2Na on antibacterial activity , which was related to the concentration of lysozyme .On E.fae-calis, the antibacterial activity of lysozyme with EDTA-2Na was 1.2-3.7 folds than the pure lysozyme when the concentration of lysozyme was 0.5-50 g/L (P0.05). Conclusion: For E.faecalis and P.endodontalis, a low concentration of lysozyme with EDTA-2Na showed significant synergistic antibacterial activity , while a high concentration of lysozyme with EDTA-2 Na did not .
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Heavy ions are the positively charged nuclei of the atomic number greater than two in the universe, which mainly comes from the charged particles of the solar system outside. Heavy ions occupy only 1% in cosmic ray, but because they are is capable of forming the Bragg peak, resulting in huge energy deposition, they have much more severe biological effects on immune organ than electromagnetic radiation in the cosmic rays. This paper outlines the biological effects of heavy ions on several aspects of immune organs, immune cells and chromosomes, and provide a theoretical basis for the study of the impact of heavy ion radiation on the immune system.
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Objective To investigate the effect of asarone on the zebrafish embryonic development and behavior. Methods The 3 hours post fertilization(hpf) zebrafish embryos were exposed to different concentrations(0, 25,50,100,200 and 400 μmol/L) of asarone solution. The asarone solution was replaced each 24 h. Microscope was used to observe embryos morphology at 24,48,72 and 96 hpf, respectively. Spontaneous movements at 24 hpf, heart rate at 48 hpf, hatch rate, deformity rate and mortality rate were evaluated.The sepn1 gene expression of zebrafish in 96 hpf was detected by RT-qPCR. With Noldus tracking system the behaviors of zebrafish larvae exposed to asarone were recorded. Results After zebrafish embryos were exposed to the different concentrations of asarone, their spontaneous movements were decreased. Compared with the control group, the 100,200 and 400 μmol/L groups were significantly decreased (P<0.01). There was a significant difference(P<0.01) in 48 hpf heart rate between all asarone groups and control group. Spine curvature, pericardial edema, yolk sac edema and other deformity were observed at 72 hpf and 96 hpf. Compared with the control group, the 100, 200 and 400 μmol/L groups showed significanly decreased hatch rate(P<0.01). There was a significant difference (P<0.05) in mortality rate between the 400 μmol/L group and control group. With concentrations of asarone increasing, the speed and distance of movement of 200 and 400 μmol/L group were significantly reduced compared to the control group(P<0.05). There was a significant difference between the 200,400 μmol/L groups and the control group in activity(P< 0.01).At 96 hpf, the expression of sepn1 gene in arone groups were decreased compared to control group, especially the 100 μmol/L group(P<0.05), and the 200 and 400 μmol/L groups(P<0.01). Conclusion Asarone had teratogenic effect on zebrafish embryos and larvae ,and inhibitory effect on larvae behavior .We should be careful to use asarone in infant medication.
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@#To investigate the effects of miRNA-10b(miR-10b)on breast cancer proliferation and apoptosis in MCF-7 and MDA-MB-231, the miR-10b mimics used to increase the endogenous expression of miR-10b, and miR-10b inhibitor used to decrease the endogenous expression of miR-10b, were stably transfected into MCF-7 and MDA-MB-231 breast cancer cells. The expression level of miR-10b was determined by real-time PCR. The effects of miR-10b on proliferation were evaluated by MTT assay, while cell cycle assay and the apoptosis rate were measured by flow cytometry. Bioinformatic software was used to predict the potential targets of miR-10b, and 3′UTR luciferase reporter and qRT-PCR assay were used to verify a direct target of miR-10b. The expression levels of caspase-3 and p21 protein were measured by Western blot. Results confirmed that over-expression of miR-10b could promote the proliferation of breast cancer cells and inhibit the apoptosis by up-regulating the endogenous miR-10b, while the miR-10b inhibitor could restrain the proliferation of breast cancer cells and increase the apoptosis by reducing the endogenous miR-10b. In conclusion, miR-10b could negatively regulate the expression of caspase-3 and p21 by targeting TP53INP1, hence highlighting its potential as an oncogene in breast cancer cells.
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<p><b>OBJECTIVE</b>To study the dispersivity of baicalin solid dispersion by Raman spectrometry and infrared spectrometry in order to obtain a new and simple method in examining the dispersivity of solid dispersion.</p><p><b>METHOD</b>Baicalin solid dispersion was prepared by solvent method. The Raman spectra, infrared spectra and X-ray powder diffraction patterns of crude baicalin, PVP K30, physical mixture, solid dispersion were acquired by Raman and Fourier transform infrared spectrometers and X-ray powder diffractometry. Then the spectra were compared with each other in order to find out the differences between baicalin solid dispersion and other materials.</p><p><b>RESULT</b>The result of Raman spectrometry was that amorphous baicalin presented in the solid dispersion, which was in agreement with that of X-ray powder diffractometry.</p><p><b>CONCLUSION</b>Raman spectrometry which is fast, direct and non-destructive is an ideal method for examining the dispersivity of solid dispersion.</p>